GPS Technology for Biological Engineering
GeneGPS
Combining Design of Experiment, Accurate Empirical Measurement and Machine Learning
At its core, Atum's GPS technology enables us to test small numbers of variants for commercially relevant properties, and to rapidly create molecules that work well in the real world. We call the platform "GPS" because it works similarly to a satellite system − it helps you to navigate the best path from your current position to your destination. The GPS platform is extremely versatile and can be applied to biological engineering at many levels including vector and gene optimization for expression in particular hosts, protein engineering for development of biocatalysts, and antibody humanization and affinity maturation.
REAL WORLD PERFORMANCE
REAL WORLD PERFORMANCE
High-quality low-throughput assays more precisely mimic the conditions that your protein will experience during its lifecycle. The result is a product that performs better in the real world, from manufacturing to its end-application.
SPEED TO MARKET
SPEED TO MARKET
ATUM's efficient workflow integrates in-house-media, assay, analytical and process development expertise. This accelerates project timelines and positions your protein for fast scale-up and manufacturing.
INTELLECTUAL PROPERTY
INTELLECTUAL PROPERTY
We can help you understand the IP landscape surrounding your protein. This translates to a stronger intellectual property position, giving you freedom to operate and broader patent claims, thereby reducing the risk of product obsolescence and being quickly overtaken by your competitors.
Applying the GPS Platform
GeneGPS®
- Search Space: 10100
- Varians Per Round: ≤ 48
- Output: Reliably gene optimization for high expression in specific hosts
VectorGPS®
- Search Space: 105
- Variants Per Round: ≤ 24
- Output: Optimal vector design for expression host and application
Literature
In Silico Design of Functional DNA Constructs
ATUM's Gene Designer software enables design of novel vector combinations from genetic elements.
ProteinGPS®
- Search Space: 1020
- Variants Per Round: ≤ 96
- Output: Protein optimized across multiple attributes affecting functionality and developability
Literature
Mapping of amino acid substitutions conferring herbicide resistance in wheat glutathione transferase
Application of ProteinGPS DOE and machine learning to characterize and understand substrate specificity of glutathione transferase in wheat.
Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin
Collaboration with Pfizer using ProteinGPS to develop an aminotransferase with a 60-fold increase in activity in Vibrio fluvialis.
"Site and Mutation" − Specific Predictions Enable Minimal Directed Evolution Libraries
Collaboration with Merck illustrating application of ProteinGPS to improve activity of a transaminase in E. Coli.
AntibodyGPS®
- Search Space: 106 - 1020
- Variants Per Round: ≤ 96
- Output: Antibody optimized across multiple attributes affecting functionality and developability