====== Creating a Construct ====== * Open GeneDesigner. * Double clicking on the program icon will create a new project, containing a single (empty) new construct. * Double clicking on an existing project will open that project. ===== The Project Window ===== {{:guide:screenshot_a.jpg}} * An open project has two windows * [[ui:library_explorer | Library Explorer]]. * [[ui:construct_editor | Construct Editor]]. * The [[ui:construct_editor | Construct Editor]] is divided into two sections: * On the left, elements are displayed in the [[ui:navigation_pane | Navigation Pane]] in a hierarchical tree. * On the right, elements are displayed in the [[ui:workspace_pane | Workspace Pane]] in a linear arrangement. ===== Adding a New Construct to a Project ===== * Select "add a new construct" from the "Add" button on the bottom left corner of the Construct Editor. You will be prompted to choose * a construct name * whether your construct is circular or linear * a codon table * add notes, if any {{:guide:screenshot_b.jpg}} ===== Adding Elements to a Construct ===== === Adding new elements === * New sequence elements can be added to a construct by dragging an element out of the New Elements Tab in the [[ui:library_explorer | Library Explorer]] and into the desired position within the construct. * When dragging in a new DNA element you will be prompted to type / paste a name and DNA sequence for the new element. * When dragging in a new amino acid (AA) element you will be prompted to type / paste a name and amino acid sequence for the new element. * When dragging in a new open reading frame (ORF) element you will be prompted to type / paste a name and DNA sequence for the new element. You will also be prompted to select a reading frame and the DNA sequence will be automatically translated. * New sequence elements can also be added to a construct using the "Add" button at the bottom of the Construct Editor. Elements will be created within the "Elements Not in a Construct" section of the Navigation Pane, and may then be dragged into the desired position within the construct. === Adding existing elements from the Library Explorer === * Sequence elements can be added to a construct from the [[ui:library_explorer | Library Explorer]] by dragging existing elements out of the [[ui:Design_toolbox | Design Toolbox]] and into the desired position within the construct. * Dragging a folder from the [[ui:Design_toolbox | Design Toolbox]] will add all of the elements within that folder as a [[ui:groups | group]] within the construct. {{:guide:screenshot_c.jpg}} === Adding sequences from other files on your computer === * Dragging a fasta file from elsewhere on your computer into the desired position within the construct. * If the fasta file contains more than one sequence, the sequences will be added as a [[ui:groups | Group]] * Dragging a genebank file from elsewhere on your computer into the desired position within the construct. * If the genbank file contains more than one sequence, the sequences will be added as a [[ui:groups | Group]] * Sequence elements can be dragged into either the [[ui:navigation_pane | Navigation Pane]] or the [[ui:workspace_pane | Workspace Pane]]. ===== Manipulating Elements within a Construct ===== * This can be done within the [[ui:icon_view | Icon View]] ===== Saving a Construct into your Library ===== * A construct or sequence element can be saved for use in other projects by dragging its icon from the [[ui:navigation_pane | Navigation Pane]] to the [[ui:library_explorer | Library Explorer]]. * A construct or sequence element can be added to an existing folder in the [[ui:Design Toolbox]] or [[ui:Vectors]] window. * A new folder may be created in the [[ui:Design Toolbox]] or [[ui:Vectors]] window by selecting the green "Add" button at the bottom of the Library Explorer * A construct or sequence element that has been saved into the [[ui:library_explorer | Library Explorer]] will be available for use in any new construct.