====== Open Reading Frame Search ======
(link to screenshot 12)
GeneDesigner permits you to search for open reading frames that exist within a construct.
 


===== Searching for open reading frames in a sequence =====
In [[ui:Sequence View]] select the "Find" panel (label in screenshot 12)
  * Select the "ORF" tab
    * Select the codons to use as start codons
    * Select the minimum ORF size to consider
    * Select the codons to use as stop codons
    * Click the "Find" button
      * Results are displayed in a hit list, which displays the position of each ORF found
      * Clicking on the ORF in the hit list 
        * moves the display to the start codon and 
        * highlights the ORF within the [[ui:Sequence View | local sequence window]]
  * Initiate a new search by clicking on the "clear" button

=== Restricting your search ===
  * The default settings are to search for ORFs within every construct in the project
  * To search for ORFs in a single construct, or just a part of a construct, select the "Restrict Search" button (label in screenshot 12).
    * Selecting this button will restrict the search to constructs or elements that are highlighted in the [[ui:navigation_pane | Navigation Pane]]. 
  

=== Viewing open reading frames in sequence view ===
  * An overview of ORFs within a construct can be obtained in the [[ui:Sequence View | Global and Local Sequence Views]] by checking the "ORF Graphical Display" box (label in screenshot 12).
    * This graphical display can be exported as a part of a [[guide:create report | report]].