====== Details View ====== * The Details View allows you to view and edit parameters that are associated with a construct. These are * Whether the construct is circular or linear * The position of the origin of a circular construct * The backtranslation profile * The Details View also allows you to generate reports about your construct ===== Backtranslation Summary Report ===== * The Backtranslation Summary provides a text file for any construct within a project, containing: * DNA sequence of * the whole construct * each element * A translational map of each Amino Acid element * A list of occurrences of motifs and restriction sites that were set to avoid during backtranslation * A list of repeats greater in length than those used during backtranslation * GC content of the construct * Notes {{:ui:backtranslation_summary.jpg}} ===== Viewing / Editing Construct Parameters ===== === Converting between linear and circular === * A construct can be converted from circular to linear by right clicking on the construct icon and selecting "convert to linear". (Not yet working in beta version) * The origin of the circle will become the left-most base in the linear construct. * A construct can be converted from linear to circular by right clicking on the construct icon and selecting "convert to circular". (Not yet working in beta version) * The left-most base in the linear construct will become the origin of the circle. * A construct can be converted from circular to linear by right clicking on the "Edit" button in the Details View and then checking (or unchecking) the "Circular" box. {{:ui:details_view_1.jpg}} === Changing the position of the origin === * The origin of a circular construct must be at the beginning of one of its component sequence elements. * The origin can be changed by by right clicking on the construct icon and selecting "change origin". (Not yet working in beta version) * You will be prompted to select the sequence element that you would like to begin at the origin. * Beta version workaround: convert the circular construct to a linear construct, rearrange the elements so that the desired element is at the left end and convert back to circular. === Viewing the backtranslation profile === * Backtranslation in GeneDesigner uses a genetic algorithm to avoid getting stuck in suboptimal local minima. * Backtranslation profiles can be created and edited from the "Configure" pull down menu at the top of the [[ui:Construct Editor]] * The backtranslation profile allows you to control * The genetic algorithm * The codon bias table and threshold * Unwanted sequences that will be excluded from backtranslated sequences, including * restriction sites * cryptic splice sites * ribosome binding sites * The size of repeats that the software attempts to eliminate. * By storing backtranslation profiles you can use the same parameters for many constructs without having to re-enter the same data every time. * More details on backtranslation can be found [[guide:backtranslate | here]] {{:ui:backtranslation_profile_used.jpg}} == Profile differences == * The Details View will indicate whether there are differences between the profile applied to a construct, and a profile that may have the same name in the profile manager. * This is intended to avoid confusion when * Two people share a construct and may have different backtranslation profiles with the same name * A backtranslation profile has been edited, but the new values apply only to that construct.