====== Sequence View ====== * The sequence view allows you to see the details of the design sequence. ===== The Sequence Navigation Windows ===== * There are two movable windows to allow navigation through through the sequence: === The Global Sequence Window === * The global sequence window (label in screenshot 3) allows selection of a large area of the construct * In a circular construct, the global window can straddle the origin. * The size of the global window can be altered by dragging one of the open squares at its boundary (label in screenshot 3). * The location of the global window can be altered by: * clicking anywhere within the selected part of the sector and dragging it. * clicking anywhere on the construct: the click point will become the center of the window. === The Local Sequence Window === * The local sequence window (label in screenshot 3) allows base-by-base navigation through the sequence * The local window can be moved in the same way as the global window. * The sequence within the local window is displayed (label in screenshot 3). {{:ui:sequence_view2.jpg}} ===== The Sequence Display ===== * At the top of the sequence display, the sequence of the final construct is shown. * Below the construct sequence is a grid where [[guide:create oligonucleotides | oligonucleotides]] are created and displayed. * Below the oligonucleotide grid, the sequence of each element is displayed in a sequence bar. Above the sequence bar is the name for that element and an arrow denoting its orienation. * The sequences for DNA elements and amino acid elements are displayed differently: === Display of Amino Acid element sequence === * The amino acid sequence bar shows the amino acid sequence of the element * Beneath each amino acid, each of the codons encoding that amino acid are displayed * The codons are shown in the order of their frequencies in the codon table selected for the construct * The highest frequency codon is at the top * The codon that is currently selected to encode each amino acid is colored * Codons whose frequencies are higher than the threshold frequency are colored green. * Codons whose frequencies are lower than the threshold frequency are colored yellow. * The threshold frequency can be changed in the [[ui:Details View]] * A new codon may be selected to encode any amino acid by clicking on a different codon button. * The new choice changes the sequence displayed for the final construct. * The new choice changes the sequence of any oligonucleotide that overlaps the change. * Clicking on the codon that is already selected will clear that codon choice and leave a gap in the final construct sequence. * For amino acid elements that are in the reverse direction, the codons are shown in the forward direction beneath the amino acid * underneath a methionine in a reverse direction amino acid element the codon is displayed as ATG * the sequence of the final construct above the methionine is shown correctly as CAT {{:ui:sequence_view3.jpg}} === Display of DNA element sequence === * The DNA sequence bar shows the DNA sequence of the element * Beneath each triplet, each encoded amino acid is displayed * There are 6 different amino acid sequences shown, 3 in the forward reading frames and 3 in the reverse. * The amino acid one letter code is shown in line with the middle nucleotide of the codon. * The direction of the reading frame is indicated by a faint arrow. * If a DNA element is adjacent to an amino acid element, the reading frame that is in the same frame as the amino acid element will be colored to match that element. ===== Editing Sequence Elements within the Sequence View ===== * The sequence of individual elements within the final construct can be [[guide:edit | edited]] in the Sequence View.