DNA2.0® Gene Design and Synthesis
ATUM is building on DNA2.0’s reputation for rapid, reliable, and accurate DNA synthesis. Using our proprietary AI/ML based GeneGPS® and VectorGPS® platforms we design constructs optimized to express in your system, whether that’s a single gene in E. coli, a metabolic pathway in yeast or a complex bispecific antibody in mammalian cells.
YIELD
YIELD
CONTROL
CONTROL
PRECISION
PRECISION
GeneGPS: ATUMS’s AI/ML based gene optimization:
ATUM’s industry leading codon optimization algorithm GeneGPS is different, proprietary, empirically based, and it works! GeneGPS eliminates bias, incorrect assumptions and flawed optimization strategies by focusing on what matters – what has been experimentally demonstrated to improve expression!
GeneGPS begins with a training set of systematically varied synthetic gene variants, whose expression is experimentally tested in a target host. The expression data is analyzed using machine learning(AI/ML) to develop a unique gene design algorithm based on ML models for that host. Genes designed using GeneGPS reliably outperform genes designed using alternative methods.
The GeneGPS technology is protected by issued US patents 7561972, 7561973, 8126653, 8401798 and pending applications.
We have built GeneGPS optimization algorithms for many bacterial, yeast, insect, plant, fungal and mammalian expression hosts. We continue to develop GeneGPS for novel expression hosts.
Gene variants optimized by GeneGPS express up to 10-fold more protein in E. coli than variants created using competitors’ algorithms (high CAI, matching host bias, etc.).
Gene optimization without vector optimization is to leave the job half done. Vector elements can improve protein solubility, reduce aggregation, direct proteins to the correct compartment, mediate genetic stability and contribute to host cell health, as well as increasing expression yield.
Our extensive Vectorology® collection of vector elements include promoters, terminators, genetic insulators, RNA processing sequences, secretion signal sequences, solubility and affinity tags, selectable markers and bacterial and eukaryotic origins of replication. With VectorGPS we combine these Vectorology elements systematically, test the resulting vectors experimentally, and use machine learning analysis of the data to identify the best combinations of elements.
We have developed many ready-to-use expression vectors with superior performance in a wide range of hosts. We continue to apply VectorGPS to design custom vectors for novel hosts and applications.
ATUM expression vectors typically outperform commercially-available vectors (pCDNA3.4, pTT5) for the transient expression of GFP in HEK293 (v1, v2), ExpiCHO (v5, v6) and in both HEK293 and ExpiCHO (v3, v4).
Coupling gene optimization and vector design give you the greatest chance of obtaining a highly performing-expression construct.
A 5-fold improvement in titer of antibody in HEK293 delivered by combining vector and gene optimization.
ATUM provides the fastest and most reliable gene synthesis turnaround time in the industry. From idea to bench in as little as 5 days to meet your research deadlines. Guaranteed.
Follow the status of your gene synthesis in real-time directly in your online ATUM account.
Priority Service: Move your genes to the top of the queue at every step in the gene synthesis process. Not quite as fast as our signature RUSH service, but genes generally ship 20% faster than standard delivery. Actual delivery times are based on the individual gene and cloning characteristics.
Restrictions: ATUM′s turnaround guarantee is not valid for orders requiring custom cloning, low copy vectors, genes encoding high GC, stretches of homopolymers, extensive repeats or genes longer than 3kb. Turnaround times can be affected if a gene is toxic to E.coli at propagation.
Orders placed before 5:00pm PST (on USA business days) will be considered ordered on the same day.
Shipping: All orders are shipped UPS Overnight from California to arrive Worldwide in 48 hours or less. US and Canadian customers can expect genes to be delivered the day after Ship date. European, Australian and Asian customers can expect 1-2 days for shipment to arrive.
Sunny California: ATUM never offshores or outsources any gene synthesis to third parties. Rest assured that all your genes are made in sunny California, 100% accurate and intellectual property compliant.