PPS-9010 (BG10, wild type): Pichia pastoris wild type expression strain. This strain has been characterized by full genomic sequencing and 5000+ gene transcriptome data sets generated for various growth conditions, including shake flask and fermentation cultures. In fermentation, BioGrammatics has achieved cell densities in excess of 700 grams wet cell pellet / liter with this strain.

PPS-9011 (BG11, aox1Δ (MutS)): A slow methanol utilization deriviative of PPS-9010. The AOX1 open reading frame has been deleted from ATG to stop. This strain grows slowly when methanol is used as the sole carbon source, and is most useful for fermentation optimization studies.

PPS-9016 (BG16, pep4Δ, prb1Δ): A protease deletion strain, derived from PPS-9010. The genotype of BG16 is pep4Δ, prb1Δ. Protease deficient strains have been shown to be effective in reducing the degradation of some foreign proteins (White et al. 1995; Brierley 1998). This is especially noticeable in fermenter cultures of secreted recombinant proteins, because the combination of high cell density and lysis of a small percentage of cells results in a relatively high concentration of these vacuolar proteases. Unfortunately, these protease deficient cells are not as vigorous as wild-type strains with respect to PEP4. In addition to lower viability, they possess a slower growth rate, are more difficult to transform, and are more difficult to handle in shake flask and fermenter cultures. Therefore, the use of protease-deficient strains is only recommended in situations where other measures to reduce proteolysis (e.g., addition of carrier compounds such as case amino acids and/or peptone to the culture medium) have yielded unsatisfactory results. In particular, it is recommended that a wild-type P. pastoris strain and not a protease-deficient strain be utilized as the initial strain for expression studies.

These strains have been jointly developed by BioGrammatics Inc. and ATUM.

Proteins and peptides made with ATUM catalog Pichia strains (the “Pichia Strains”) can be used commercially without license obligations. However, neither the Pichia Strains, nor any strains derived from the Pichia Strains, may be transferred or sold to third parties, resold, modified for resale, or used to provide a service of any kind to third parties, including, without limitation, reporting the results of customer activities for a fee or other form of consideration. See the complete legal Terms and Conditions here.