Protein Expression Services

Our protein services combine expression optimization, purification expertise and comprehensive protein analytical capabilities to ensure that your protein is made according to your precise specifications. Whether you need protein made in bacterial, yeast or mammalian hosts, we can help.

QUALITY

Activity and purity come first. Always. Our expertise in expression and purification combined with state-of-the-art protein analytics ensures that every protein that we produce is of the highest quality.

SCALE

From 96-well plates to multiple liters, delivering up to 10's of grams of purified protein from bacterial, yeast and mammalian expression hosts.

SPEED

DNA synthesis through to protein purification 'all under one roof' enables us to rapidly complete your orders, whatever the size, in as little as 4 weeks.

Types of Molecules

Every year, we purify many thousands of different proteins. If your protein is challenging because of poor expression, low solubility or a tendency to aggregate, we can help.

Higher titers start with better genes. Just provide us with the amino acid sequence of the protein to be expressed. We will design and optimize a gene and clone it into a vector for protein expression in:

Whether you need micrograms of many different proteins for your discovery program, or grams of highly purified protein for animal studies, we have the production scale and purification strategy to match. Our cell cultures range from 96-well plates of 1ml cultures through shake flasks to bioreactors delivering grams of proteins. Expression testing/piloting, desalting, custom buffer formulations, and aliquoting are all available on request.

Typical Titers

Distribution of yields of recombinant human IgG1 antibody produced by ATUM between January and May 2018. Culture volume was 50-100 ml.

*discoCHO & miCHO are CHO-K1 derived cell lines

Discovery with manufacturing in mind!

Struggling with unpredictable cell line productivity and CQAs in biologics manufacturing? Discover how ATUM’s discoCHO platform revolutionizes the process by seamlessly integrating discovery hits into our CHO-K1-based stable miCHO-GS platform—derisking your path to manufacturing success.

ATUM offers a range of analytical services to characterize the identity, purity, quantity and quality of your protein. Our tools include gel electrophoresis, HPLC (reverse phase, ion exchange and size exclusion), mass spectrometry, bio-layer interferometry (ForteBio Octet Red384 and HTX systems) and bacterial endotoxin testing. We can design a customized analytical package appropriate for your molecule and subsequent applications including IND filing and GLP toxicology studies.

ATUM has extensive experience in measuring functional properties of proteins. This can be binding of an antibody to its antigen, the activity of an enzyme under specific physical conditions or other properties that make the protein useful.

Your purified protein is shipped overnight frozen or on ice packs as requested. A comprehensive report for every analytical service performed is uploaded electronically to your account. Download sample analytical reports:

Expertise