NEWARK, Calif.– In this free webinar, learn about transposase-mediated genome engineering, genetic quality control and ensuring the genetic stability of orthogonal transposons in the generation of cell lines with Leap-In Transposase® technology.
Discover a groundbreaking webinar delving into transposase-mediated genome engineering, genetic quality control (QC) and stability of orthogonal transposons in the generation of cell lines with Leap-In Transposase® technology. Genes encoding biotherapeutic proteins can stably be introduced into mammalian genomes by several different approaches. The transposon-based platform avoids major challenges presented by random integration or viral-based delivery systems.
Here the experts from ATUM will demonstrate how they can use the Leap-In Transposase® technology to genetically engineer Chinese hamster ovary cells (CHO) cells multiple times to manipulate and control their expression profile. By sequentially using three different and orthogonal Leap-In Transposase® enzymes, they have generated a panel of CHO cell lines where they modulate their metabolism to aid selection, integrate a biotherapeutic protein and modify its glycosylation profile to produce stable and high-expressing cell lines with a desired product quality attributes.
To ensure that each transposon is integrated correctly and to demonstrate its orthogonality, they have performed genetic QC by means of Targeted Locus Amplification (TLA) coupled with next-generation sequencing (NGS). This allowed for the identification and genetic characterization of all integration sites of each transposon. Furthermore, by comparing the TLA data of different timepoints they were able to evaluate the genetic stability and integrity of the transposons.
Join this webinar to learn about transposase-mediated genome engineering, genetic quality control and ensuring the genetic stability of orthogonal transposons in the generation of cell lines with Leap-In Transposase® technology.
Join Maya Wright Clark, PhD, Sales and Account Manager, Cergentis; and Mário Pereira, Director of Technologies and Business Development, ATUM, for the live webinar on Thursday, November 9, 2023, at 10am EST (4pm CET/EU-Central).
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