1. Select Target
Gene
Name
Gene
Locus
Heterologous
DNA
2. Results
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gRNA
3. Delivery Options
Select
Vector
Download
Oligos

CRISPR gRNA Design tool

Order your tandem gRNAs in the All-in-one NickaseNinja™ construct for increased transfection efficiency.
to

ACGT only. Maximum of 10,000 bp. 


												
											

											
											

												Non-encoding/special characters
											

											
											

												
											

						 				

						 			

					 			

				 			

				 			
				 			
				 			
					 		

					 			
					 			
					 			

									
									

										
										
									

								

								
					 			
Enter your gRNA in FASTA format:

								
								

									

									
								

								
								

									Non-encoding/special characters 
									SapI restriction site
								

								
								
									This tool currently accepts 20 bp gRNA's for cloning into a Cas9 vector. 
									If you would like to use ATUM's NickaseNinja™ vector with tandem custom gRNAs, please email us at
									info@atum.bio
								
								
								

									
								

					 		

					 	

			
						
					

				

			

		

		
		
		
		
		
	
    
    

    	

		    

		    	
**Disclaimer**:

		    	

			    	
			    		The ATUM gRNA Design Tool is provided "as is", with no explicit or implicit guarantees for any purpose.
			     		ATUM cannot guarantee the performance of any individual gRNA designed using the Tool.
			     	
			     	
			     		Custom CRISPR Constructs and CRISPR Catalog Vectors are not for use in humans.
			     	
			     	
						Please see our Terms & Conditions 
						for additional information and complete disclaimer language. Thank you.
					
				

		    
	
    	

    

    
		
	

				

			

		


		

			

				

					

						

							
						

						
©  ATUM. All Rights Reserved.

						
Made in sunny California.

						
					

                    

                        


                            

                                

                                    
                                        
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