Protein Analytics
We offer a range of protein analytic services to characterize the identity, purity, quantity and quality at every stage of your protein – from discovery all the way to manufacturing. Every protein purified at ATUM includes the following standard analytical package: appearance description, quantitation by A280, molecular weight and purity analysis by micro-capillary electrophoresis, and aggregation analysis by SEC-HPLC.
The standard package can be augmented with other protein analytical services. We can work with you to build an analytical package appropriate for your molecule and subsequent applications (e.g., IND filing, toxicology studies).
Our Protein Analytics Offerings
Below is a menu of our current analytical offerings.
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Overview
Discovery / Engineering
Titer measurements
- HPLC
- Octet
Epitope binning*
- Octet
Identity & Purity
- SEC-HPLC
- µCE-SDS
- MS Identity*
Hydrophobicity*
- RP-HPLC
- HIC-HPLC
Charge heterogeneity*
- cIEX-HPLC
- cIEF
Aggregation propensity*
- AC-SINS
- DLS (Hydrodynamic radius, kD, A2)
Thermostability
- Tm (DSF)
- Tagg (DLS)*
Polyspecificity
- BVP-ELISA
Stability
- pH stress
- Thermal stress
- Freeze-thaw stress
- Agitation stress
Readout:
- SEC-HPLC
- µCE-SDS
Manufacturability*
- Formulation study
- Concentration study
Readout:
- SEC-HPLC
- µCE-SDS
- DLS (Hydrodynamic radius, kD, A2)
- HCP Analysis
- HCPDNA Analysis
- Glycan Analysis
Activity*
Binding kinetics
- Octet, SPR
- ELISA
Cell based Assays
FcgRI, FcRn interaction
- Octet, SPR
Enzyme kinetics
Developability
See case studies on:
mAbs developability analytics
in silico predictions
- Mol. wt.
- Isoelectric point (pI)
- N-Glycans
- Hydrophobicity-GRAVY
- Cysteine count
- Sequence liabilities (deamidation, isomerization, acid labile)
- Epitope analysis
Stability
- pH stress
- Thermal stress
- Freeze-thaw stress
- Agitation stress
Readout:
- SEC-HPLC
- µCE-SDS
Afucosylation
See case studies on Afucosylation Analytics
Titer measurement (Harvest supernatant)
- HPLC
- Octet
Standard QC (purified protein)
- SEC-HPLC
- CE-SDS
Glycan analysis (purified protein)
- HILIC-HPLC
Activity (purified protein)
- ADCC (pseudo)
- SPR binding
Bispecifics/Multispecifics Analytics Overview
See case studies on Bispecifics Analytics
Titer measurement (Harvest supernatant)
- HPLC
- Octet
Standard QC (purified protein)
- SEC-HPLC
- CE-SDS
Heterodimer % (purified protein)*
- HIC-HPLC
- cIEX-HPLC
Activity (purified protein)*
- Octet
- ELISA